1. Research Paper Journal of Sericulture, 1999, Vol.7(1&2):30-32
STUDIES ON THE REELING PERFORMANCE OF COCOONS DAMAGED BY UZI MAGGOT
H. B. MAHESHA AND S. HONNAIAH
ABSTRACT
Cocoons from three varieties [Pure Mysore, NB4D2 and Cross breed] were collected from grainages and cocoon market. Both normal and uzi infested cocoons were reeled using epprouvette at low speed. Filament length, number of breaks, loss of silk, reelability and denier were determined both for damaged and normal cocoons. The reeling of uzi infested cocoons was found to be unsuitable for producing quality silk.
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2. Research Paper Indian J. Seric., 2000, Vol. 39, No.2, 139-144
HAEMOLYMPH PROTEINS OF F1 PROGENY RAISED FROM ETHYL METHANESULFONATE TREATED SILKWORM BOMBYX MORI L.
H. B. MAHESHA, P. H. THEJASWINI AND S. HONNAIAH
ABSTRACT
Two races of mulberry silkworms namely Pure Mysore and NB4D2, at the age of fifth instar first day were treated with 2.5, 5 and 10 mM EMS by oral injection. The F1 progeny was obtained by selfing the moths emerged from the EMS treated silkworms. The quantitative analysis of haemolymph proteins of F1 progeny revealed that the silkworm batches treated with lower doses like 2.5 and 5 mM exhibited higher levels when compared to their respective control sets. The qualitative analysis was also clearly indicated the variations in the number as well as intensity of the protein bands.
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3. Research Paper Ann. Entomol. 18 (1):27-32 (2000)
EFFECT OF CYTOPLASMIC POLYHEDROSIS ON HAEMOLYMPH PROTEINS OF SILKWORM BOMBYX MORI L.
MAHESHA H. B., THEJASWINI P. H. AND HONNAIAH S.
ABSTRACT
The effects of infection by a cytoplasmic polyhedrosis virus on the haemolymph proteins in two races of silkworm Bombyx mori were studied. In the diseased larvae, the quantity of proteins gradually decreased as the disease progressed. The electrophoretic analysis also revealed variation in the number as well as intensity of protein bands. Such changes in the infected larvae, dipicting the possible defense cellular adjustment of the host in response to viral attack.
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4. Research Paper J. Seric., 2002, 8-10 (1&2): 38-43
EFFECT OF CYTOPLASMIC POLYHEDROSIS ON SILKWORM (BOMBYX MORI L.) AND ITS TRANSMISSION
FROM GENERATION TO GENERATION
H. B. MAHESHA AND S. HONNAIAH
ABSTRACT
The LC50 for larval mortality during fifth instar was 0.096 X 106 cytoplasmic polyhedral bodies per ml in Pure Mysore and 0.218 x 106 PIBs per ml in NB4D2 silkworms. Besides mortality of silkworms, there was a significant reduction in cocoon weight, shell weight, and shell ratio in the surviving silkworms. The F1 progeny obtained by selfing the mothsemerged from the silkworms inoculated with BmCPV exhibited inferiority in fecundity, hatchability, larval weight, cocoon yield, cocoon weight, shell weight, shell ratio, filament lengt denior, and effective rate of rearing when compared to control batches.
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5. Research Paper Entomon., 2002, 27(3):269-279
EFFECT OF CYTOPLASMIC POLYHEDROSIS ON AMYLASE AND SUCCINATE DEHYDROGENASE ACTIVITY LEVELS IN SILKWORM BOMBYX MORI L.
H. B. MAHESHA, P. H. THEEJASWINI AND S. HONNAIAH
ABSTRACT
Two races of mulberry silkworm namely Pure Mysore and NB4D2, at fifth instar first day were inoculated with two different doses of cytoplasmic polyhedrsl inclusion bodies viz., 1.562 x 106/ml and 3.125 x 106 /ml by oral injection. the effects of cytoplasmic polyhedrosis on the amylase and succinate dehydrogenase activity levels in haemolymph as well as midgut tissue were studied. in the diseased larvae, the enzyme activity levels were gradually decreases as the disease progressed. such changes in the infected larvae, depicts the possible defense callular adjustment of the host in response to pathogen attack.
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6. Research Paper Indian J. Seric., 2002, 41(1): 24-28
AMYLASE AND SUCCINATE DEHYDROGENASE ACTIVITY LEVELS IN F1 PROGENY RAISED FROM ETHYL METHANESULFONATE TREATED SILKWORM BOMBYX MORI L.
H.B.MAHESHA AND S. HONNAIAH
ABSTRACT
Two races of mulberry silkworm namely, Pure Mysore and NB4D2 at fifth instar first day were treated with 2.5, 5 and 10 mM ethyl methanesulfonate by oral injection. The F1 prrogeny was obtained by selfing the moths emerged from the EMS treated silkworms andcontrol sets separately. The activity levels of amylase and succinate dehydrogenase in haemolymph and midgut tissue of F1 progeny at fifth instar was estimated.The results clearly indicated that the silkworm batches obtained from the EMS treated larvae exhibited almost similar pattern with altered levels of enzyme activity when compared to their respective control sets.
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7. Scientific Paper Sericologia., 2002, 43(3):343-360.
FFECTS OF ETHYL METHANESULFONATE ON MEIOTIC CHROMOSOMES OF SILKWORM BOMBYX MORI L.
H. B. MAHESHA AND S. HONNAIAH
ABSTRACT
The effects of ethyl methanesulfonate (EMS), on meiotic chromosomes in general and translocations in particular was studied during spermatogenesis in two races of silkworm Bombyx mori L. Different concentrations of EMS like 2.5, 5 10, 20 and 40 mM in 0.75 % NaCl was employed by "oral injection". The results indicated that increase in concentration EMS would enhance the frequency of abberation in total as well as individual types. They includes loops, patrially paired bivalents, fragments, stickyness and, stickyness and clumping. The basic knowledge could be useful in silkworm breeding programme.
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8. Research paper Indian J.Seric., 2009, 48(2), 126-132
EFFECT OF NUCLEAR POLYHEDROSIS ON SOME BIOMOLECULES OF SILKWORM BOMBYX MORI L.
H.B. MAHESHA, H. P. KRUPA AND P.H.THEJASWINI
ABSTRACT
The effect of infection by nuclear polyhedrosis on the haemolymph proteins, amylase, superoxide dismutase and beeta esterase were studied in the silkworm Bombyx mori L. in the diseased larvae, the quantity of total proteins were gradually decreased as the disease progressed. The electrophoretic analysis also revealed the variation in the number as well as intensity of protein bands. Also, isozymes of amylase, superoxide dismutase and beeta esterase exhibited variation in the R.F. and intensity, in addition to the appearence of a new fraction of superoxide dismutase. Such changes in the infected larvae, depicting the possible defense cellular adjustment of the host in response to viral attack.
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9. Conference Abstract - National Conference on Recent Trends in Animal Physiology, October 2009, UOM
STUDIES ON INDUCTION OF RESISTANCE AGAINST NUCLEAR POLYHEDROSIS IN SILKWORM BOMBYX MORI L AND ITS BIOCHEMICAL ASPECTS
H.B. MAHESHA AND P.H. THEJASWINI
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10. Research Paper J. Entomological Res., 3(1):43-50
A study on straight and reciprocal crossing in F1 hybrids using bivoltine and multivoltine silkworm, Bombyx mori L. (Lep., Bombycidae) races
E.Talebi, M. Khademi, G.Subramanya, H.B. Mahesha
Abstract
The effects of straight and reciprocal crossing on F1 hybrids were estimated on six quantitative traits viz., cocoon weight, shell weight, shell ratio, filament length, denier and renditta in the silkworm, Bombyx mori L. utilizing two bivoltine races and two multivoltine races namely NB4D2, C108, Nistari and Pure Mysore, respectively. In order to understand the genetics of cross breeding system between multivoltine and bivoltine races, a hybridization experiment was conducted by crossing females of multivoltine races (Pure Mysore and Nistari) with males of bivoltine races (C108 and NB4D2) and its reciprocals during 2009. The twelve straight and reciprocal hybrids were reared under standard laboratory condition. The data of the hybrids was analysed for the estimation of six economical traits to study the crossing effects, between the hybrids. The straight and reciprocal crossing of C108 × NB4D2 has shown maximum cocoon weight (0.37 gr) whereas PM × Nistari has recorded minimum cocoon weight (0.2 gr) among the hybrids. Superiority of strait crosses between multivoltine and bivoltine races only have been shown for denier trait in PM × NB4D2 hybrid. In bivoltine × multivoltine hybrids, characters such as cocoon weight, shell weight, filament length and denier showed high significant difference.
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11. Research Paper Asian J.Exp.Biol.Sci., 2012, 3(2): 642-653
Correlation studies on haemolymph and midgut tissue proteins with commercial characters of
silkworm Bombyx mori L.
Farshid Ghasemi Kasmaei and H.B.Mahesha
ABSTRACT
Four mulberry silkworm races viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore × CSR2 and Nistari × NB4D2 ) silkworms were selected for the present study. The total soluble protein present in the haemolymph as well as midgut tissue was estimated. The qualitative analysis of total protein was carried out by SDS-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, single cocoon weight, single shell weight, shell ratio, filament length, denier and renditta were selected. They were subjected for statistical analysis to know the level of correlation between the biomolecules and commercial characters. The results clearly showed that haemolymph protein has positive correlation with selected commercial characters except larval duration and renditta. Also the midgut protein indicated positive correlation except fecundity, larval weight, shell ratio and renditta. The SDS-PAGE analysis also revealed the variations in protein profiles of the experimental sets.
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12. Research paper Annals Biol.Res., 2012, 3(7):3518-3532
Analysis of correlation between haemolymph and midgut tissue amylase with commercial characters of silkworm Bombyx mori L.
Farshid Ghasemi Kasmaei and H.B.Mahesha
ABSTRACT
Four mulberry silkworm races viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore × CSR2 and Nistari × NB4D2 ) silkworms were selected for the present study. The specific activity of amylase in the haemolymph as well as midgut tissue was estimated. The qualitative analysis of amylase was carried out by Native-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were selected. The results of quantitative analysis were subjected for statistical analysis against selected commercial characters to know the level and kind of correlation between them. The results of statistical analysis clearly showed that haemolymph amylase has highly positive correlation with selected commercial characters except larval duration. In contrast, the midgut amylase indicated highly positive correlation with larval duration only. The zymograms of amylase also exhibited variation among the selected silkworm varieties.
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13. Research paper Annals Biol.Res., 2012, 3(7):3638-3651
Studies on succinate dehydrogenase and its relationship with economic characters of silkworm Bombyx mori L.
Farshid Ghasemi Kasmaei and H.B.Mahesha
ABSTRACT
Four mulberry silkworm races viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore × CSR2 and Nistari × NB4D2 ) silkworms were selected for the present study. The specific activity of succinate dehydrogenase (SDH) in the haemolymph as well as midgut tissue was estimated. The results of quantitative analysis were subjected for regression analysis against selected commercial characters viz., fecundity, larval weight, larval duration, single cocoon weight, single shell weight, shell ratio, filament length, denier and renditta, to know the correlation coefficient between them. The results of statistical analysis clearly showed that haemolymph and midgut SDH activity level has positive correlation with selected commercial characters except renditta. The qualitative analysis of SDH was carried out by Native-PAGE. The zymograms of succinate dehydrogenase also exhibited variation among the selected silkworm varieties.
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14. Research paper Annals Biol.Res., 2012, 3(11):5273-5292
Studies on the esterase and its relationship with commercial characters of silkworm Bombyx mori L.
Farshid Ghasemi Kasmaei and H.B.Mahesha
ABSTRACT
Four pure mulberry silkworm breeds viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore x CSR2 and Nistari x NB4D2 ) silkworms were selected for the present study. The specific activity of esterase in the haemolymph, midgut and fat body tissues was estimated. The qualitative analysis of esterase was carried out by Native-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were selected. The esterase activity levels were subjected for regression analysis against selected commercial characters to know the level and kind of correlation between them. The results of statistical analysis clearly showed that the haemolymph esterase activity levels exhibited positive correlation with denier, filament length, larval duration, shell ratio, cocoon weight and shell weight. The midgut esterase activity levels showed positive correlation with denier, fecundity, filament length, larval weight, shell ratio, cocoon weight and shell weight. Also, the activity levels of fat body esterase indicated positive correlation with denier, fecundity, filament length, larval weight, shell ratio, cocoon weight and shell weight only. The zymograms of esterase also exhibited variation among the selected silkworm breeds.
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15. Research paper Annals Biol.Res., 2012, 3(11):5259-5272
Analysis of alkaline phosphataseand its relationship with commercial characters of silkworm Bombyx mori L.
Farshid Ghasemi Kasmaei and H.B.Mahesha
ABSTRACT
Four pure mulberry silkworm breeds viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore x CSR2 and Nistari x NB4D2 ) silkworms were selected for the present study. The specific activity of alkaline phosphatase (ALKP) in midgut and fat body tissues were estimated. The qualitative analysis of ALKP was carried out by Native-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were selected. The activity levels of ALKP of midgut and fat body tissue showed statistically significant (P<0.001) changes in their activity levels during fifth instar. The results of quantitative analysis were subjected for regression analysis against selected commercial characters to know the correlation coefficient between them. The results of regression analysis clearly showed that midgut ALKP has positive correlation with fecundity, larval duration and renditta only. The activity levels of fat body ALKP showed positive correlation with fecundity, larval weight and renditta only. The zymograms of ALKP in midgut and fat body also exhibited variation among the selected silkworm varieties.
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16. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(5):1071-1082
Analysis of correlation between commercial characters of silkworm Bombyx mori L.
Mahesha H B, Ghasemi K F and Thejaswini P H
ABSTRACT
Six pure silkworm breeds viz., Pure Mysore, Nistari, C. nichi, NB4D2, KA & CSR2 and three hybrid (Pure Mysore x CSR2, Nistari x NB4D2 and KA x NB4D2 ) silkworms were used for the present study. The silkworm rearing was conducted during pre monsoon, monsoon and post monsoon seasons to get the mean values of selected commercial characters. The mean values of commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were subjected to regression analysis with each other individually to know the level of correlation coefficient between them. Of the 36 analysis, 21 traits clearly showed the positive correlation.
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17. Research paper IJBPAS, 2013, 2(5):1093-1102
Effect of ethyl methanesulfonate on viability and commercial characters silkworm
Bombyx mori L. ans their inheritance
Mahesha H B, Ghasemi K F and Thejaswini P H
ABSTRACT
Two races of mulberry silkworm namely Pure Mysore and NB4D2, at the age of fifth instar first day were treated with different doses of ethyl methanesulfonate (EMS) by oral injection. The treated larvae along with their respective controls were allowed to continue development. Silkworms of both the races exhibited gradual reduction in the viability as the dose of EMS increased. The F1- F4 progenies were obtained by selfing the moths emerged from the EMS treated silkworms and control sets separately. The experimental results clearly indicated that the silkworm batches treated with lower doses like 2.5 and 5 mM of both the races exhibited improvement in commercial characters when compared to their respective controls as well as 10 mM sets. In addition, the altered characters exhibited regular inheritance from generation to generation.
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18. Research Paper Int. J. Pure App.Biosci.,2013, 1(3):11-16 Studies of the haemolymph glucose level in silkworm Bombyx mori during cytoplasmic polyhedrosis
H.B. Mahesha and P.H. Thejaswini
ABSTRACT
Two mulberry silkworm breeds viz., Pure Mysore and NB4D2 were selected for the present study. The breed susceptibility was determined during fifth instar by oral injection method. Two doses of cytoplasmic polyhedral inclusion bodies i.e., 1.562 x 106 and 3.125 x 106 were selected based on the breed susceptibility test and inoculated in to silkworm larvae by oral injection. The control as well as inoculated silkworm larvae were collected daily with a regular interval of 24 h for haemolymph collection. The collected haemolymph was subjected for glucose assay. Among the experimental batches, the diseased larvae exhibited reduction in the blood glucose level and also there was gradual reduction as the disease progressed. Such changes in the infected larvae, depicting the possible defense cellular adjustment of the host in response to viral attack.
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19. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(7):1501-1512
Studies on induction of tolerance against nuclear polyhedrosis in silkworm
Bombyx mori L. and its biochemical aspects
Mahesha H B, Rahamathulla G and Thejaswini P H
ABSTRACT
To induce tolerance in silkworm against nuclear polyhedrosis virus, two sets of experiments were carried out. For both the experiments four mulberry silkworm breeds namely NB4D2, CSR2, Pure Mysore, C. nichi and a commercial hybrid (PM X CSR2) were used. In the first set of experiments silkworms were fed with known amount of formalin killed Bombyx mori nuclear polyhedral inclusion bodies (PIBs) thrice at various time intervals for multiple immunization. In the second set of experiments such treatment was given only once. At the age of 5th instar second day the silkworms of all the batches were challenged with known amount of live PIBs. Among immunization methods, multiple immunization method was more effective as all the races indicated by high survival rate. Also, variation in quantitative biochemical composition was observed with respect to haemolymph proteins and, enzymes such as succinate dehydrogenase and amylase activity levels when compared with the unimmunized controls. The results pave the way towards the use of immunization method to induce tolerance in silkworm against the dreaded nuclear polyhedrosis virus.
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20. Research Paper Asian J. Exp. Biol.Sci. Vol 4(3) 2013:437-449
Studies on the fat body biomolecules and their relatonship with economic traits of silkworm Bombyx mori L.
H.B.Mahesha and Farshid Ghasemi Kasmaei
ABSTRACT
Studies on the fat body biomolecules and their relatonship with economic traits of silkworm Bombyx mori L.
H.B.Mahesha and Farshid Ghasemi Kasmaei
ABSTRACT
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21. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(7):1526-1534
Studies on the correlation between DNA, RNA and Proteins of Bombyx mori L.
Mahesha H B, Kasmaei F G and Thejaswini P H
ABSTRACT
Four pure mulberry silkworm breeds viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore x CSR2 and Nistari x NB4D2 ) silkworms were selected for the present study. The total DNA, RNA and soluble proteins present in the mid alimentary canal was estimated during fifth instar with a regular interval of 24 h, and the average concentration during fifth instar was calculated. The average concentration of DNA, RNA and proteins were subjected to regression analysis with each other individually to know the kind and level of correlation coefficient between them. The results of regression analysis between DNA and RNA as well as between RNA and Protein exhibited very strong level of correlation coefficient between them. The average concentration of DNA and protein exhibited moderately strong positive relationship between them. The concentration of DNA, RNA and protein as well as their ratios are more in hybrid silkworms.
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22. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(5):1071-1082
Analysis of correlation between commercial characters of silkworm Bombyx mori L.
Mahesha H B, Ghasemi K F and Thejaswini P H
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23. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(8):1665-1677 Studies on the nucleic acids and and their correlation with economic between commercial characters of silkworm Bombyx mori L.
Kasmaei F G and Mahesha H B
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24. Research Paper Int. J. Pure App.Biosci.,2013, 1(4):37-41 Haemolymph glucose level of F1 progeny raised from ethyl methanesulfonate treated silkworm Bombyx mori L.
H.B. Mahesha and P.H. Thejaswini
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25.National Symposium on Innovations in Science and Technology for Inclusive Development
University of Mysore, 3-4 January 2014
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26.National Symposium on Innovations in Science and Technology for Inclusive Development
University of Mysore, 3-4 January 2014
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27. Research Paper Int. J. Pure App. Biosci.,2014, 2(2):48-55 Studies on isozymes of amylase, superoxide dismutaes and esterase during induction of tolerance against nuclear polyhedrosis in silkworm Bombyx mori L.
H.B. Mahesha and P.H. Thejaswini
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28. Research Paper Int. J. Pure App.Biosci.,2015, 3(2):54-62
Elicitation of induced resistance in pearl millet against Sclerospora graminicola through seed treatment with medicinal plant extracts
P.H. Thejaswini, H. Shekar Shetty and H.B. Mahesha
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29.
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30. Research Paper Int. J. Pure App.Biosci.,2015, 3(2):173-180 Studies of the correlation between protein, amylase, succinate dehydrogenase, esterase and alkaline phosphatase of silkworm Bombyx mori L.
H. B. Mahesha, Farshid G. K. and P. H. Thejaswini
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31. Research Paper Int. J. Pure App. Biosci.,2015, 3(2):413-418
Comparative studies on the midgut proteins during cypovirus infection and in mutants raised from EMS treated silkworm Bombyx mori L.
H.B. Mahesha and P.H. Thejaswini
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32. Research paper Int. J. Biol. Phar. Allied Scie., 2013, 2(8):1665-1677 Effects of induced tolerance against nuclear polyhedrosis on economic characters of silkworm Bombyx mori L and their inheritance Bombyx mori L.
Mahesha H B and Thejaswini P H
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33. Research Paper Int. J. Pure App. Biosci.,2013, 6(2):725-734 Histological aspects of disease resistance in pearl millet against Sclerospora graminicola
H.B.Mahesha, D.S. Prathima and P.H. Thejaswini
ABSTRACT
Two cultivars of Pennisetum glaucum viz., 7042S and IP18294, highly susceptible and highly resistant to virulent pathotype 1 of S. graminicola, and a virulent pathotype 1 of S. graminicola was used in the present investigation. The commonly available medicinal plants, viz., Cymbopogan citrates, Zingiber officinale, Trigonella corniculata, Cicca acida, Murraya koenigii were grinded with distilled water, acetone, chloroform and methanol at 4 oC and extract was used as inducer of resistance. After preliminary studies like seedling vigor, vegetative and reproductive growth parameters, selected inducer in solvent was used to induce the resistance in plants by seed treatment. In the experimental sets, the histological parameters like host tissue necrosis, quantum of lignin, tannin, suberin and phenolics were tested. The results clearly indicated that the curry leaf extract induced remarkable resistance when compared to other experimental sets. Thus the results of this study contribute to development of a promising method to effectively inoculate the seeds with required inducer of resistance.
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34.
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35.
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